TABLE 1.
Estimation of pseudodimer effects
| Pseudodimer fraction
|
||||
|---|---|---|---|---|
| Parameter | 0% | 5% | 10% | 15% |
| k1 (× 105/M/s) | 7.04 (1.00) | 8.88 (1.26) | 10.1 (1.43) | 13.2 (1.88) |
| k2 (× 107/M/s) | 5.48 (1.00) | 5.69 (1.04) | 5.57 (1.02) | 6.56 (1.20) |
| k3 (× 108/M/s) | 2.16 (1.00) | 2.01 (0.93) | 1.98 (0.92) | 1.81 (0.84) |
| Predimers (per cell) | 448 (1.00) | 424 (0.95) | 406 (0.91) | 353 (0.79) |
Fitting to the model shown in the text was carried out assuming 0–15% of Rh-EGF dimers in Fig. 5 D were pseudodimers constructed by two Rh-EGF accidentally bound to monomeric EGFRs existing nearby. See text for the meaning of each parameter. Numbers in parentheses are ratios with respect to the numbers at 0% pseudodimer.