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. 2026 Mar 12;14(4):e01915-25. doi: 10.1128/spectrum.01915-25

Fig 1.

Flowchart showing neutrophil gene analysis from RNA-seq of two subtypes to 1255 co-genes, screening via differential expression and GO enrichment to 20 candidates, qPCR verification yielding a 4-gene TB signature evaluated in two external cohorts.

Experimental workflow of the study. The study design comprised five independent cohorts. RNA sequencing of two neutrophil subtypes revealed 1,255 overlapping genes (co-genes). Differential expression analysis (DEGs) and Gene Ontology (GO) enrichment analysis identified 20 candidate genes. A 4-gene signature derived from qPCR validation was further assessed in two external cohorts.