TABLE 2.
Ion conductance of α-hemolysin
| pH | bias (V) | Itotal (pA) | Ntotal | ICl (pA) | NCl | IK (pA) | NK | Simulation time (ns) |
|---|---|---|---|---|---|---|---|---|
| 8.0 | 1.2 | 1360 | 44.1 | 490 | 15.9 | 870 | 28.2 | 5.23 |
| 8.0 | 0.6 | 700 | 48.8 | 300 | 19.7 | 400 | 28.9 | 11.22 |
| 8.0 | 0.24 | 280 | 20.2 | 140 | 11.0 | 140 | 9.2 | 10.56 |
| 8.0 | 0.12 | 130 | 6.3 | 70 | 3.5 | 60 | 2.8 | 8.92 |
| 8.0 | 0.0 | −10 | 0.0 | 20 | 1.4 | −30 | −1.4 | 8.30 |
| 8.0 | −0.12 | −110 | 8.0 | −10 | 1.6 | −100 | 6.4 | 11.02 |
| 8.0 | −0.24 | −160 | 11.8 | −90 | 8.2 | −70 | 3.6 | 12.44 |
| 8.0 | −1.2 | −950 | 30.0 | −580 | 17.7 | −370 | 12.3 | 4.96 |
| 4.5 | 1.2 | 1460 | 50.3 | 600 | 21.5 | 860 | 28.8 | 5.42 |
| 4.5 | 0.6 | 720 | 27.8 | 330 | 13.0 | 390 | 14.8 | 6.42 |
| 4.5 | 0.24 | 200 | 13.6 | 130 | 8.8 | 70 | 4.8 | 11.04 |
| 4.5 | −0.24 | −280 | 17.3 | −140 | 8.3 | −140 | 9.0 | 10.81 |
| 4.5 | −0.6 | −390 | 13.5 | −330 | 9.8 | −60 | 3.7 | 6.00 |
| 4.5 | −1.2 | −520 | 26.8 | −390 | 19.6 | −130 | 7.2 | 7.29 |
The average currents were computed by applying a linear regression fit to the cumulative current curves (cf. Fig. 11). The number of ion permeations was computed by dividing the total charge transported through the channel by the unitary charge (1.6 × 10−19C).