FIGURE 3.

Oxidation effects on the WT ClC-0. (A) Effects of 10 μM CuPhe on ClC-0. All current traces in the left, middle, and right panels were from the same oocyte. Recordings on the left were made after the oocyte was treated with 5 mM β-ME for 1 h. Recordings in the middle were made after the oocyte was treated with 10 μM CuPhe. At right are recordings made after the oocyte was again treated with 5 mM β-ME after the CuPhe treatment. The number of crossovers and the current at the most depolarized voltage (both presented as mean ± SD) for 20 oocytes are shown. The asterisks indicate a significant difference from the corresponding value on the left panel (pair t-test with the significance level at p = 0.01). (B) Recordings of WT ClC-0 in another 19 oocytes. The recording procedure was the same as that shown in A, except that the recordings in the middle panel were made after 1 h of treatment with 10 μM CuPhe + 5 mM β-ME. (C) Effects of CuSO₄ on WT ClC-0. The recording procedure was the same as that shown in A and B, except that the recordings in the middle panel were made after 1 h of treatment with 10 μM CuSO₄ (n = 7). (D) Effects of H₂O₂ on WT ClC-0. The experimental procedure was the same as those shown in A–C, except that the recordings in the middle panel were made after 1 h of treatment with 0.01% H₂O₂ (n = 5).