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. 2005 Mar 18;88(6):3936–3945. doi: 10.1529/biophysj.104.055012

FIGURE 7.

FIGURE 7

Effects of oxidation on the current induction of the K165C heterodimer by MTSET and MTSEA. (A and B) Induction of current in the K165C heterodimer by 30 μM MTSET and 3 μM MTSEA in oocytes without (left) or with (right) β-ME pretreatment. Each circle represents the current amplitude measured at the end of the +40-mV voltage step in protocol 1. (Insets) Whole oocyte current recordings before (indicated by b) and after (indicated by a) the application of MTS reagents. Each trace is an average of three traces made during the period indicated by short line segments. (C and D) Ratios of the current amplitudes of the heterodimer before and after the treatment of MTS reagents in oxidized (−β-ME) and reduced (+β-ME) conditions. Note that in the reduced condition (also see right panel in both A and B), the current of the oocyte after MTSEA treatment is ∼2-fold that before MTSEA induction (Ia/Ib ≅ 2). MTSET did not induce current (Ia/Ib ≅ 1) in reduced oocytes.