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Dependence of the specific activity of 5-LO on the content of anionic lipids in fluid (POPC/POPG) and solid (DPPC/DPPG) membranes. Total lipid concentration was 350 μM in all cases, AA concentration was 100 μM, and 5-LO was added to a final concentration of 2.4 μg/ml. The buffer, method of 5-LO activity measurement, and other experimental conditions are described in Materials and Methods.
