FIGURE 5.

Isotherms of 5-LO binding to vesicles composed of DPPC (•), POPC (▪), PLPC (▴), PAPC (□), and PDPC (▵). The data points are obtained on the basis of the decrease in Trp fluorescence emission intensity due to energy transfer from Trp to Py-PE (2 mol % in vesicle membranes), as measured in RET experiments (e.g., Fig. 4). The theoretical curves are simulated through Eq. 1 using binding parameters summarized in Table 1.