TABLE 1.
Parameters characterizing the interaction of 5-LO with large unilamellar vesicles composed of PCs of varying fluidity
| Lipid | L1/2 (μM) | KD (μM) | N |
|---|---|---|---|
| DPPC | 443.5 | 1.15 | 184 |
| POPC | 297.8 | 0.78 | 176 |
| PLPC | 199.2 | 0.56 | 157 |
| PAPC | 83.2 | 0.24 | 127 |
| PDPC | 92.0 | 0.32 | 114 |
Parameters include lipid concentrations corresponding to binding of 50% of 5-LO (L1/2), the dissociation constants (KD), and the numbers of lipids per 5-LO binding site (N). Lipids were composed of phosphatidylcholines with varying degrees of sn-2 acyl chain unsaturation.