TABLE 3.

Effect of 2 M sucrose on the phosphorescence lifetime (τ^S) and on the acrylamide quenching rate constant () of azurin species, at 20 and 40°C

Protein	T (°C)	τB (s)	τS (s)	τS/τB†		(M−1s−1)	(M−1s−1)
WT	20	0.63	0.66	1.05	1.10	32	1.0
C3AC26A	20	0.47	0.52	1.06	1.13	180	1.0
F110S	20	1.10*	1.87*	1.70	3.53	8.9 × 104	1.6
I7S	20	0.16*	0.20*	1.25	1.40	5.0 × 105	1.8
WT	40	0.117	0.114	1.17	1.26	3.5 × 102	1.5
C3AC26A	40	0.101	0.120	1.20	1.30	1.0 × 103	1.4
F110S	40	0.080*	0.261*	3.20	5.20	2.8 × 105	3.0
I7S	40	0.023*	0.035*	1.52	1.83	1.1 × 106	2.5

^*Average lifetimes from a biexponential fit of the decay.

^†Effective viscosity derived from the empirical relationship between τ and bulk solvent viscosity in model compounds (Strambini and Gonnelli, 1995).