Table 3.
Diversity estimates and results of tests statistics for selective sweeps
| Sequenced fragment | Gene | No. of silent sites | No. of individuals | Nucleotide diversity, π | Tajima's D* | Fu and Li's D | Divergence | P value, H test | P value, HKA test† |
|---|---|---|---|---|---|---|---|---|---|
| Africa | |||||||||
| frag.1 | period/CG2650 | 861 | 32 | 0.0178 | −0.569 | −0.446 | 0.058 | 0.452 | — |
| frag.2 | 100G10.2 | 781 | 28 | 0.0169 | −0.677 | −1.007 | 0.074 | 0.368 | 0.66 |
| crm‡ | cramped | 1210 | 7 | 0.0233 | −0.199 | 0.411 | 0.117 | 0.234 | 0.305 |
| syx4 | syxntaxin4 | 657 | 25 | 0.0072 | −0.750 | −1.352 | 0.071 | 0.241 | 0.101 |
| frag.3 | Noncoding | 1279 | 16 | 0.0122 | −0.596 | −1.077 | 0.063 | 0.38 | 0.414 |
| frag.4 | Noncoding | 824 | 11 | 0.0076 | −0.741 | −1.146 | 0.045 | 0.551 | 0.392 |
| frag.5 | Noncoding | 992 | 14 | 0.0127 | −0.765 | −1.041 | 0.074 | 0.2462 | 0.372 |
| frag.6 | CG3588 | 459 | 14 | 0.0325 | −0.191 | −0.039 | 0.135 | 0.1752 | 0.693 |
| frag.7 | CG3592 | 431 | 18 | 0.0278 | 0.587 | −0.769 | 0.155 | 0.6409 | 0.343 |
| Europe | |||||||||
| frag.1 | period/CG2650 | 861 | 30 | 0.0090 | 0.122 | −0.161 | 0.059 | 0.099 | — |
| frag.2 | 100G10.2 | 781 | 28 | 0.0062 | −0.135 | 0.699 | 0.073 | 0.070 | 0.295 |
| crm | cramped | 1210 | 27 | 0.0030 | −0.730 | 1.824§ | 0.120 | 0.003 | 0.001 |
| syx4 | syxntaxin4 | 657 | 29 | 0.0009 | −0.338 | −0.296 | 0.074 | 0.064 | 0.002 |
| frag.3 | Noncoding | 1279 | 28 | 0.0036 | 1.270 | 0.651 | 0.066 | 0.100 | 0.015 |
| frag.4 | Noncoding | 824 | 13 | 0.0052 | 1.229 | 0.549 | 0.048 | 0.440 | 0.322 |
| frag.5 | Noncoding | 992 | 10 | 0.0012 | 0.131 | 0.915 | 0.073 | 0.050 | 0.003 |
| frag.6 | CG3588 | 459 | 9 | 0.0026 | −0.788 | −0.806 | 0.147 | 0.093 | 0.005 |
| frag.7 | CG3592 | 431 | 12 | 0.016 | 1.01 | 1.634¶ | 0.159 | 0.114 | 0.212 |
Tajima's D was in all loci nonsignificant (P > 0.05).
HKA test for all fragments was performed with frag.1 as the control locus.
Two fragments covering the fixed differences between African and non-African flies (≈800 bp each) were sequenced in an additional 22 African individuals (Kenya and Zimbabwe) to confirm that the intronic and the replacement substitutions were fixed between African and non-African flies.
P (Fu and Li's D) < 0.02.
P (Fu and Li's D) < 0.05.