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Experimental data of CaMKII and eGFP-CaMKII activation by wild-type and A633(C2)CaM shown in Fig. 3, fitted with the Michaelis-Menten equation (Eq. 30)
| Protein combination | Vmax [μmol/min/mg] | Kact [μmol] |
|---|---|---|
| wtCaM/wtCaMKII | 10.54 ± 0.97 | 0.056 ± 0.010 |
| wtCaM/eGFP-CaMKII | 9.55 ± 1.12 | 0.106 ± 0.016 |
| A633(C2)CaM/wtCaMKII | 7.18 ± 0.79* | 0.106 ± 0.030 |
| A633(C2)CaM/eGFP-CaMK | 7.84 ± 1.43* | 0.189 ± 0.063 |
Statistically significant p < 0.05.
