TABLE 3.

Summary of binding parameters of eGFP-CaMKII and A633(C2)CaM in HEK cells under varying conditions seen in Fig. 5

Binding parameter	+ Ca2+	+ Ca2+/MgATP	+ EGTA
r	0.35	0.31	(0.3)*
Θ	1.0	1.0	<0.08
Kd [μM]	(<0.02)*	(<0.002)*	>36
eGFP-CaMKII
Cfree [nM]	0	0	142
Ctotal [nM]	304	222	399
CaM
Cfree [μM]	4.8	0.23	3.2
Ctotal [μM]	8.4	2.9	3.6

The parameter r represents the fraction of labeled CaM to account for the presence of additional unlabeled CaM in the cell. The given concentration values include the labeled and unlabeled molecules of the respective species.

^*Assumption.