. 2005 Mar 18;88(6):4337–4350. doi: 10.1529/biophysj.104.057497

TABLE 1.

The steady-state fluorescence spectrum parameters of LAURDAN in AOT-isooctane reverse micelles as a function of w₀

	Total emission					Log-normal Gaussian No. 1					Log-normal Gaussian No. 2					Log-normal Gaussian No. 3
w₀	I_m	ν_m (10⁴cm⁻¹)	λ_m (nm)	FWHM (10⁴cm⁻¹)	QY	I_m₁	ν_m₁ (10⁴cm⁻¹)	λ_m₁ (nm)	FWHM₁ (10⁴cm⁻¹)	QY₁	I_m₂	ν_m₂ (10⁴cm⁻¹)	λ_m₂ (nm)	FWHM₂ (10⁴cm⁻¹)	QY₂	I_m₃	ν_m₃ (10⁴cm⁻¹)	λ_m₃ (nm)	FWHM₃ (10⁴cm⁻¹)	QY₃
0	1.883	2.410	415	0.2922	0.239	0.214	2.608	383	0.873	0.007	1.054	2.443	409	0.2237	0.096	1.013	2.345	426	0.3082	0.136
2	1.658	2.375	421	0.3419	0.263	0.228	2.603	384	0.925	0.008	1.381	2.396	417	0.2771	0.160	0.543	2.217	451	0.3498	0.095
5	1.254	2.364	423	0.3922	0.235	0.237	2.597	385	0.986	0.009	1.236	2.372	422	0.3136	0.167	0.358	2.078	481	0.2933	0.059
8	1.076	2.364	423	0.4319	0.212	0.224	2.587	387	0.1060	0.009	1.064	2.368	422	0.3120	0.144	0.355	2.058	486	0.2898	0.059
11	0.949	2.364	423	0.4563	0.194	0.218	2.584	387	0.1098	0.009	0.941	2.370	422	0.3213	0.132	0.323	2.045	489	0.2848	0.054
20	0.806	2.375	421	0.4490	0.172	0.237	2.578	388	0.1210	0.010	0.802	2.376	421	0.3192	0.112	0.289	2.040	490	0.2878	0.050
33	0.706	2.381	420	0.4558	0.157	0.239	2.577	388	0.1236	0.011	0.701	2.382	420	0.3197	0.100	0.254	2.038	491	0.2916	0.046

The parameters result from their log-normal Gaussian deconvolution (see Materials and Methods). Quantum yield (QY) was calculated taking 1,1,4,4-tetraphenylbutadiene in cyclohexane as the reference compound (quantum yield of 0.60; Berlman, 1971). The QY for each emitting species is the QY for total emission weighted by the emitting surface spectrum of that species. A sum of three elemental log-normal Gaussian curves (see Materials and Methods) was used for the deconvolution.