Skip to main content
. 2002 Sep 19;99(20):12991–12996. doi: 10.1073/pnas.192219599

Figure 4.

Figure 4

A HindIII RFLP of F8A fragments distinguishes hemophilia A, carrier female, and normal dog genomic DNA. The 28- and 13-kb HindIII bands found in normal dog genomic DNA (N) are not found in hemophilia A dog DNA (H), which demonstrates bands at 21 and 23 kb. The carrier female (C) has all four bands seen in hemophilia A or normal dog DNA. The normal dog BAC clone 291 M9 has the 28-kb band, suggesting the 28-kb fragment is extragenic to factor VIII. Normal dog BAC clones 292 C4 and 314 O16 contain the 13-kb band, suggesting the 13-kb fragment is within factor VIII, presumably in intron 22 where these BACs overlap. Not shown are bands at ≈5 and ≈3.5 kb that are present in all three genomic DNA samples (see Fig. 11).