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. 2002 Sep 23;99(20):13014–13019. doi: 10.1073/pnas.202323999

Figure 4.

Figure 4

Evidence for a functional PreB/stromal cell synapse. (A) Double staining of Nalm6 preB cells cultivated on MS5.1 using anti-P-Tyr and the anti-human IgM mAbs. m, merge view. (B Upper) Diagram representation of the frequency of Nalm6-relocalized cells for 5-, 30-, and 120-min coculture on MS5.1. For each point, 300–500 stromal-adhering preB cells were examined, and the results are expressed as percent of total lymphoid cells. (Lower) CD79a proteins were immunoprecipitated from Nalm6 lysates after coculture with MS5.1. Immunoprecipitates were blotted with anti-CD79a, anti-P-Tyr, and anti-VpreB mAbs after separation on an SDS/7.5–15% PAGE under reducing conditions. (C) CD79a proteins were immunoprecipitated from Laz221 lysates and analyzed as in B. In addition, CD79a proteins were immunoprecipitated after 30 min of coculture in the presence of scSLC* (80 μg/ml) or lactose (0.1 M). Control includes immunoprecipitation with an isotype-matched (M2) mAb.