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. Author manuscript; available in PMC: 2006 Oct 1.
Published in final edited form as: Am J Physiol Regul Integr Comp Physiol. 2005 Jun 9;289(4):R1155–R1168. doi: 10.1152/ajpregu.00234.2005

Figure 5.

Figure 5

Fluorescence images of P2X2 (A-F) or P2X3 (G-L) receptor expression in the suburothelial plexus (A-C; G-I) and serosa (D-F; J-L) of adult animals. Whole mounts were also stained for protein gene product (PGP9.5) (B,E,H,K) to confirm that P2X2 and P2X3-immunoreactive structures were nerve fibers. In all tissues examined, the P2X2 and P2X3-IR in the suburothelial plexus or serosa was colabeled with PGP9.5. Merged images (C,F,I,L) demonstrating overlap between P2X2 and PGP9.5 (C,F) or P2X3 and PGP9.5 (I,L). Some isolated nerve fibers demonstrating P2X2- and PGP9.5-IR (C, arrow) or P2X3-and PGP9.5-IR (I, arrow) are shown in the suburothelial plexus. Calibration bar represents 50 μm.