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. 2002 Sep 13;99(20):13125–13130. doi: 10.1073/pnas.202480499

Figure 1.

Figure 1

Inhibition of NQO1 activity induces p53 and p73 proteasomal degradation. (A) HCT116 cells were incubated for 4 h without (−) or with 200 or 400 μM dicoumarol (Dic.) and without (−) or with (+) 100 μM MG 132. (B) M1-t-p53 cells were preincubated at 32°C without (−) or with (+) 10 nM thapsigargin or 40 μM calpeptin for 1 h followed by culture for an additional 5 h without (−) or with (+) 400 μM dicoumarol. (C) A31N- ts20 cells were transfected with 200 ng of pRc/CMV human wild-type p53 alone or cotransfected with 1.5 μg of pSUPER RFX (Su-RFX) or pSUPER NQO1 (Su-NQO1) with and without 1 μg of pEFIRES-HA-NQO1. The cells were cultured at 32°C. Levels of human p53 were determined 24 h after transfection.