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Diagnostic performance of the culture-free method, compared with the culture method (A) Accuracy stratified by smear status, culture status, and patient group. (B) Accuracy by pretreatment protocol (acid vs. NALC-NaOH). NTM, nontuberculous mycobacteria; AFB, acid-fast bacilli; NALC-NaOH, N-acetyl-L-cysteine–sodium hydroxide.
