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. 2002 Sep 16;99(20):13154–13159. doi: 10.1073/pnas.192432299

Figure 2.

Figure 2

Affinity purification of baculovirus expressed NS3 with immobilized GST-p11 and colocalization of NS3 and p11 in BTV-infected cells. (A) Radiolabeled clarified lysate from Sf9 cells infected with the recombinant baculovirus expressing NS3 (lane 1) was incubated in the presence of GST alone (lane 2) or GST-p11 (lane 3). Wild-type baculovirus lysates were also incubated in the presence of GST-p11 as a control (lane 4). (B) BHK cells were infected with BTV and the colocalization of NS3 and p11 proteins was visualized by incubation with anti-NS3 antiserum (1:1,000) or/and anti-p11 antiserum (1:5,000), as primary antibodies followed by incubation with secondary antibodies labeled with FITC (green) or tetramethylrhodamine isothiocyanate (red) respectively. Immunofluorescent staining of NS3 (Top); immunofluorescent staining of p11 (Middle); dual staining of both NS3 and p11 proteins in infected cells (Bottom).