Figure 2.

Hippocampal neurons and microglial cells do not respond to Reelin in the stripe-choice assay. (a) Neurons that migrated out from a hippocampal slice (P4) onto the stripe matrix after 14 DIV (red, Reelin-coated stripes). Cell bodies and neurites, stained with the mAb TUJ1 and a green fluorescent secondary antibody, do not show a preference for either the Reelin-coated stripes or the control stripes. (Stripe width, 90 μm.) (b) A neuron is shown (slices from P4, 14 DIV) that had migrated out of a hippocampal slice and is located on a Reelin-coated stripe. Note that neurites have grown on both the Reelin stripe and the control stripe. (Stripe width, 90 μm.) (c) Migrating cells on the stripe matrix were stained with GSA I-B₄, a marker for microglial cells (slice from P4, 14 DIV). The location of these migrating cells does not reflect the striped pattern of the coated matrix. Thus, microglial cells do not show a preference for either the Reelin-coated stripes or the control stripes. (Bar, 100 μm.) (d) Detail showing two migrating cells stained with GSA I-B₄. (Bar, 10 μm.)