Skip to main content
. 2026 Apr 8;12(15):eaec4368. doi: 10.1126/sciadv.aec4368

Fig. 5. SLC13A2 regulates global protein acetylation and transcriptional programs in HCC.

Fig. 5.

(A) Total protein acetylation in liver tissues from HTVi mice with liver-specific overexpression or KO of SLC13A2 (n = 3). (B) Immunoblot of subcellular fractions from Hepa1-6 cells overexpressing SLC13A2, probed with pan-acetyl-lysine antibody. (C) Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of proteins lacking detectable acetylation sites identified by acetyl-proteomics in SLC13A2-OE Hepa1-6 cells. (D) GO molecular function enrichment analysis of proteins with detectable acetylation sites identified by acetyl-proteomics in SLC13A2-OE Hepa1-6 cells. (E) Histone modification levels in HTVi liver tumor tissues of control and SLC13A2 LKO mice detected by immunoblotting. Data are presented as means ± SEM (n = 3). *P < 0.05; **P < 0.01, versus controls, two-tailed unpaired Student’s t test. (F) Immunoblot analysis of histone acetylation marks in Hepa1-6 cells overexpressing SLC13A2. The data are representative from three independent experiments. (G) Immunoblot analysis of histone acetylation marks in Hepa1-6 cells transfected and treated with increasing concentrations of citrate (0, 250, and 500 μM) for 48 hours. (H) Volcano plot of hepatic genes in SLC13A2-OE mice. (I) Heatmap of representative genes involved in cell proliferation and metabolism. (J) GSEA of RNA-seq showing gene enrichment in SLC13A2-OE mice. (K) Heatmap of key TFs in SLC13A2-OE mice. NES, Normalized Enrichment Score.