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. 2002 Sep 20;99(20):13217–13221. doi: 10.1073/pnas.172504199

Figure 3.

Figure 3

Modulation of glutamate-stimulated CREB phosphorylation by Aβ. A shows the phosphorylation of CREB at Ser-133. Aβ alone has no consistent effect on basal pCREB levels. Phosphorylation is stimulated strongly by treatment with 50 μM glutamate (Glut) for 15 min. Pretreatment with 5 μM Aβ for 2 h results in a 40–45% decrease in pCREB (Aβ + Glut). This effect is completely opposed by the addition of 3 μM rolipram to the preincubation mixture (Aβ + Rol + Glut). Neither rolipram nor Aβ by themselves had a significant effect on basal pCREB levels. Ctrl, control. B shows the same membranes reprobed with a phosphorylation-insensitive antibody for CREB (1:1,000, Santa Cruz Biotechnology).