Figure 6.

Nitrotyrosine content in neutrophils. (A) Isolated PMN were incubated with vehicle (control) or one of the LXA₄ analogues for 15 min and then with LPS (1 μg/ml) for 4 h at 37°C. Cells were lysed and nitrotyrosine content was determined by an enzyme immunoassay. Values are means ± SEM (n = 7). *, P < 0.05; **, P < 0.01 (vs. LPS). (B) Nitrotyrosine Western blots from neutrophil extracts. Note the increased tyrosine nitration of several proteins by LPS and inhibition with LXA₄ analogues. Tyrosine nitration by ONOO⁻ (80 μM) treatment is shown as a positive control. Blot is representative of five separate experiments.