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. 2026 Mar 3;8:39. doi: 10.1186/s42523-026-00539-8

Table 2.

The physiological, hematological, biochemical parameters and rumen volatile fatty acid during thermoneutral and heat stress exposure in zebu calves

Physiological Parameters
Parameter Thermoneutral Heat Stress SEM P Value
Rectal Temperature (° C) 38.579 39.324 0.047 0.001
Respiration rate (Breathes /Minute) 16.667 30.667 2.144 0.001
PR (beats/minute) 66.667 71.333 2.666 0.141
Hematological Parameters
WBC (103/ µL) 11.217 10.367 1.322 0.126
Lymphocyte (%) 72.517 71.900 2.673 0.827
Granulocyte (%) 31.200 27.133 3.301 0.273
Red Blood Cells (Millions/µL) 8.527 7.585 0.381 0.007
Hemoglobin (g/dL) 11.033 9.600 3.804 0.004
PCV 33.067 28.050 1.156 0.005
Biochemical Parameters
AST (IU/mL) 71.879 47.432 5.489 0.007
ALT (IU/mL) 30.117 28.642 3.562 0.696
Triglyceride (mg/dL) 63.537 43.727 5.400 0.014
Urea (mg/dL) 24.597 27.277 3.236 0.445
Cortisol (ng/mL) 5.221 7.831 0.470 0.003
HSP-70 (ng/mL) 11.070 10.389 1.156 0.581
HSP-90 (ng/mL) 74.145 81.951 8.292 0.390
IL-6 (ng/mL) 40.444 48.530 3.804 0.047
TNF-α (ng/mL) 15.617 15.063 1.39 0.324
Rumen Volatile Fatty Acid
TVFA 3.408 3.2300 0.468 0.719
Acetate (%) 73.245 72.642 3.562 0.696
Propionate (%) 19.100 18.920 3.375 0.960
Butyrate (%) 7.663 8.605 3.236 0.771
A: P ratio 4.023 4.120 0.677 0.892