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Sensitivity of primers Pm1 and Pm2 in the single PCR amplification from various amounts of P. marneffei 497H DNA template and analyzed by agarose gel electrophoresis with ethidium bromide staining. Lanes: M, 100-bp-ladder DNA marker; 1 to 10, P. marneffei DNA template at 50 ng, 5 ng, 0.5 ng, 50 pg, 5 pg, 1 pg, 0.5 pg, 0.25 pg, and 50 fg and H2O, respectively.
