TABLE 3.
Nucleotide differences between the six Japanese rickettsial isolates and their closest relative speciesa
| Rickettsial isolate | Nucleotide differences by comparison with: | 16S rDNA | gltA |
|---|---|---|---|
| IP-1 | R. helvetica | No difference | No difference |
| IM-1 | R. helvetica | No difference | No difference |
| IO-1 | R. helvetica | 68 (T→C), 73 (G→A), 124 (T→C), 243 (G→A), 302 (G→T), 388 (G→A), 441 (C→T), 474 (G→T), 942 (C→T), 1059 (A→G), 1085 (A→G), 1132 (C→T), 1356 (T→A), 1361 (G→A) | 8 (G→A), 86 (A→G), 133 (A→G), 181 (T→G), 202 (A→G), 415 (T→G), 609 (C→T), 730 (T→C), 793 (T→C), 832 (T→C), 889 (G→A), 1144 (G→T) |
| FLA-1 | R. japonica | No difference | No difference |
| DT-1 | R. japonica | 749 (G→C), 766 (C→T), 1390 (C→G) | 771 (C→G), 784 (T→C), 1134 (A→T) |
| AT-1 | Slovakia 4 from I. ricinus | 37 (A→G), 44 (C→T), 45 (T→C), 91 (A→G), 162 (A→G), 163 (A→G), 688 (A→G), 693 (T→A), 815 (A→G), 972 (G→A) | 16 (C→A), 19 (G→A), 160 (A→G), 235 (A→G), 288 (G→A), 528 (A→G), 605 (A→G), 619 (G→A), 691b (none→A), 908 (T→C), 974 (A→G), 988 (A→G), 1145 (G→T) |
| AT-1 | Slovakia 3 from I. ricinus | 37 (A→G), 44 (C→T), 45 (T→C), 91 (A→G), 162 (A→G), 163 (A→G), 688 (A→G), 693(T→A), 815 (A→G), 972 (G→A), 1219 (A→none) | 16 (C→A), 160 (A→G), 235 (A→G), 288 (G→A), 528 (A→G), 605 (A→G), 619 (G→A), 691b (none→A), 974 (A→G), 988 (A→G), 1145 (G→T) |
a
Nucleotide positions are relative to those of the 16S rDNA and gltA sequences of R. helvetica (GenBank accession numbers L36212 and U59723, respectively), R. japonica (GenBank accession numbers L36213 and U59724, respectively), Slovakia 4 from I. ritinus (GenBank accession numbers AF141908 and AF141906, respectively), and Slovakia 3 from I. ricinus (GenBank accession numbers AF141907 and AF140706, respectively). Substitutions are indicated as nucleotide in reference species → nucleotide in Japanese isolate.
b
The nucleotide difference at position 691 is an insertion.