Fig. 3.

Quantitative analysis of antimicrobial activity and PC mRNA transcript copy numbers in ileal mucosa. (A) Antimicrobial activity in mucosal biopsies of ileal CD and non-IBD controls. Protein extracts were incubated with cultures of either E. coli (Left)or S. aureus (Right), and bacterial killing was assessed by using a flow cytometric assay. Biopsies from ileal CD (all NOD2 wild type) were obtained from either macroscopically inflamed (open circles) or uninflamed (filled diamonds) regions of the ileum. (B) Expression levels of four PC antimicrobials (on the left) and six nonantimicrobial PC products in ileal specimens of controls and ileal CD. Data are compiled from experiments described in Figs. 1 and 5 and are expressed as mRNA copy number per 10 ng of RNA.