Table 3. Analysis for positive antigen selection of single EBV⁺ CD27⁺ PB B cells.

	Mean		% P < 0.05†
Patient	R/SFWR*	R/SCDR*	FWR	CDR	FWR + CDR
IM1	2.2	3.2	60	60	100
IM2	1.9	2.8	80	40	50
IM3	1.7	3.7	80	40	50
IM4	2.3	4.3	83	50	60
IM5	1.5	2.7	60	20	33
IM6	2.4	3.6	67	50	75
Total	2.1	3.4‡	73	43	60

^*R/S ratios were calculated by dividing the number of R mutations by the number of S mutations in either FWR or CDR regions. For sequences where the denominator was 0, 1 was used to obtain a ratio value

^†P values were calculated by using the multinomial distribution model (24). Percent of V_H sequences having significantly fewer R mutations in FWR (P_FWR < 0.05) and percent of V_H sequences having significantly more R mutations in CDR (P_CDR < 0.05) are shown. All V_H sequences with P_CDR < 0.05 also had P_FWR < 0.05. The last column shows percent of V_H sequences with P_FWR < 0.05 also having P_CDR < 0.05

^‡Mean R/S_CDR is significantly greater than mean R/S_FWR (one-tailed Student's paired t test, P = 0.017)