FIG. 6.

Multiple-sequence alignment of N- and C-terminal domains of selected members of the 2HCT family. N-terminal halves of sequences that produced hits with C-terminal halves, and vice versa, were selected, and a multiple sequence alignment was produced with CLUSTAL W (141). The overall sequence similarity between the two groups of five N-terminal (top) and C-terminal (bottom) halves is indicated with an asterisk for identical residues and with a tilde for similar residues. The positions of the putative TMSs in the N- and C-terminal halves are indicated by solid lines above and below the sequences, respectively. Similarly, the positions of the conserved regions, Vb and Xa, are indicated by dotted lines.