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. Author manuscript; available in PMC: 2026 Apr 11.
Published in final edited form as: Biochem Biophys Res Commun. 2026 Feb 10;807:153439. doi: 10.1016/j.bbrc.2026.153439

Fig. 2.

Fig. 2.

Effects of relay domain mutations on myofibril assembly and stability in the dorsal longitudinal indirect flight muscle. A. Transverse section from EMB-D509N late-stage pupa. Myofibrils show disruption in assembly of thick and thin filaments, with gaps in the myofibril structure. B. Transverse sections from EMB-A511D and C. EMB-D509N-A511D late-stage pupae. Myofibrils show normal morphology with double-hexagonal packing of thick and thin filaments. D. Longitudinal section from EMB-D509N late-stage pupa. Sarcomeres contain gaps and frayed edges. E. Longitudinal section from EMB-A511D and F. EMB-D509N-A511D late-stage pupae. Sarcomeres appear normal with regular M – and Z-lines. G. Transverse section from EMB-D509N 2-h-old adult shows continued disruption in myofibril morphology and packing of thick and thin filaments. H. Transverse section from EMB-A511D 2-h-old adult displays disrupted myofibril morphology. I. Transverse section from EMB-D509N-A511D 2-h-old adult shows normal filament packing. J. Longitudinal sections from EMB-D509N and K. EMB-A511D 2-h-old adults show sarcomere gaps with disrupted M – and Z-lines. L. Longitudinal section from EMB-D509N-A511D 2-h-old adult shows more subtle disruption of M – and Z-lines, with fewer gaps than single mutants. M. Transverse sections from EMB-D509N and N. EMB-A511D 2-d-old adults show similar levels of disruption as 2-h-old adults. O. Transverse section from EMB-D509N-A511D 2-d-old adult shows somewhat more disruption of myofibril morphology than 2-h-old adults. P. Longitudinal sections from EMB-D509N and Q. EMB-A511D 2-d-old adults have enhanced degradation of M – and Z-lines compared to 2-h-old adults. R. Longitudinal section from EMB-D509N-A511D 2-d-old adult. Sarcomeres show disruption of filament packing, with retention of Z-lines. S. Transverse section from EMB-D509N 1-week-old adult retains structure similar to 2-d-old adult. T. Transverse sections from EMB-A511D and U. EMB-D509N-A511D 1-week-old adults show continued myofibril morphology degradation, with scattered thick and thin filaments throughout the myofibril. V. Longitudinal section from EMB-D509N 1-week-old adult shows gaps in sarcomeres with poorly defined M – and Z-lines. W. Longitudinal section from EMB-A511D 1-week-old adult displays sarcomeres that appear over contracted. X. Longitudinal section from EMB-D509N-A511D 1-week-old adult shows continued gapping and fraying, with some retention of M – and Z-lines. The myofibril shown in each panel is representative of the population at that given stage of development. Scale bars, 0.5 μm. M, M-line. Z, Z-line. m, mitochondrion.