Table 1.
Actin-sliding velocities and Ca+2, Mg+2, actin-stimulated Mg+2 ATPase kinetics of EMB-D509N, EMB-A511D and EMB-D509N-A511D myosins.
| Myosin | Actin sliding velocity ± SD (μms−1) | Basal Ca-ATPase ± SD (s−1) | Basal Mg-ATPase ± SD (s−1) | Actin-stimulated Vmax ± SD (s−1) | Km (actin) ± SD (μM) |
|---|---|---|---|---|---|
| EMB-D509N (n = 4) | 0 | 5.83 ± 0.87** | 0.10 ± 0.01*,† | 0.62 ± 0.07†† | 0.20 ± 0.08 |
| EMB-A511D (n = 5) | 0 | 5.94 ± 0.82** | 0.13 ± 0.02*** | 0.96 ± 0.15*** | 0.10 ± 0.08 |
| EMB-D509N-A511D (n = 4) | 0 | 3.76 ± 0.47 | 0.06 ± 0.01 | 0.42 ± 0.06 | 0.06 ± 0.03 |
| EMB§ | 0.7 ± 0.2 (n = 3) | 4.47 ± 2.20 (n = 6) | 0.08 ± 0.04 (n = 7) | 1.10 ± 0.38 (n = 7) | 0.29 ± 0.18 (n = 7) |
Statistically significant difference relative to EMB-D509N-A511D:
*
= p < 0.05;
**
= p < 0.01;
***
= p < 0.001.
Statistically significant difference of EMB-D509 N relative to EMB-A511D:
†
= p < 0.05;
††
= p < 0.01.
Values calculated by one-way ANOVA with Tukey’s multiple comparison test (GraphPad Prism version 10.4.2). Primary data are found in Appendix A. Supplementary data (ATPase data and statistics).
§
Data for EMB isoform [5] provided for comparison purposes.