FIG. 1.
Dot blot analysis of sheep brain homogenates for the presence of PrPSc. Serial twofold dilutions of sheep brain homogenates used for inoculation into mice were dotted onto a nitrocellulose membrane and stained with B-103 anti-PrP synthetic peptide rabbit serum and ECL Western blot detection reagent (Amersham). The sheep are indicated at the bottom and dilutions are on the right. In this dot blot analysis, S1 appears to be negative for PrPSc; however, PrPSc was detected in the immunoblot analysis (data not shown).