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Relative PK resistance of sheep PrPSc. (a) PrPSc-containing fractions prepared from sheep brains were treated with various concentrations of PK (0 to 1,000 μg/ml, indicated above the photo) at 37°C for 2 h. Molecular mass markers are indicated. PrP was detected by immunoblotting with PrP-specific antibody and ECL. (b) The same PrPSc-containing fractions as used in panel a were treated with PK (200 μg/ml) at 37°C for various times (0 to 16 h, indicated above the photo).
