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. 2005 Dec;73(12):7906–7913. doi: 10.1128/IAI.73.12.7906-7913.2005

FIG. 3.

FIG. 3.

SDS-PAGE and Western blot analysis of E. intestinalis spore protein extract before and after TFMS treatment. A Coomassie blue-stained gel of E. intestinalis spore protein extract (top panel, lane A) was deglycosylated with TFMS in the absence (lane B) or in the presence (lane C) of the control protein α-galactosidase. The patterns for the control glycoprotein alone before TFMS treatment (lane E) and after TFMS treatment (lane D) are also shown. Protein fractions A, B, and C were size fractionated by SDS-PAGE, transferred to a single PVDF membrane (bottom panel), and incubated with anti-recEiPTP1 (lanes 1, 4 and 7) and two human anti-polar tube sera (lanes 2 and 3). The molecular weight of PTP1 decreased after TFMS treatment (lanes 4 and 7), while the reactivity of the human sera was lost after TFMS treatment (lanes 5, 6, 8, and 9). The positions of molecular weight markers (in kDa) are indicated on the left (lane M).