FIG. 1.

Restriction endonuclease, plasmid, and open reading frame (ORF) maps of the laz region. The line labeled FA1090 and MC58 represents the restriction endonuclease map of a region of the N. gonorrhoeae strain FA1090 genome sequencing project (accession number AE004969) and a region of the N. meningitidis strain MC58 genome sequencing project (accession number AE002447). The open arrow above the line indicates the orientation and the location of the azurin ORF. Below the FA1090-MC58 line, the thick black lines represent the plasmids constructed during this work. The vector, pGEM T-Easy (Promega), of each of these plasmids is represented by black boxes. The restriction endonuclease sites shown indicate where the kanamycin resistance cassette (KAN) was inserted. The rectangular box at the top represents the PCR products used as probes in this study. The laz probe was constructed from PCR products utilizing primers azurinfor and H8inrev.