FIG. 3.

Expression of IP-10 and MIG mRNA and protein in lungs of wild-type (wt) and IFN-γ^−/− mice challenged with K. pneumoniae. For mRNA analysis, lungs were harvested 48 h after bacterial administration and chemokine mRNA levels were determined by quantitative real-time PCR (A). For protein analysis, lungs were harvested 48 and 72 h after bacterial administration and chemokine protein levels were determined by ELISA. For real-time PCR four lungs per group per time point were combined and averaged and represent fold increase over uninfected control. For protein analysis, n was six lungs per group per time point and data presented are a composite of two separate experiments. *, P was <0.05 compared to that for the uninfected control. **, P was <0.01 compared to that for the uninfected control or infected IFN-γ knockout (ko) mice. kp, Klebsiella-infected mice.