Time course of IFN-γ production by PBMC in response to MSP3-LSP. PBMC were stimulated for 5 days with 30 μg/ml MSP3-LSP. The results are expressed as box plots and whiskers that show the 5th, 25th, 50th, 75th, and 95th percentiles for individual IFN-γ secretion in the supernatant, expressed in pg/ml for each immunization group. The arrows indicate the times of immunization.