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. 2005 Dec;73(12):8109–8118. doi: 10.1128/IAI.73.12.8109-8118.2005

FIG. 1.

FIG. 1.

Enrichment of A. marginale outer membranes. Decreasing concentrations of 1.0, 0.2, 0.04, and 0.008 μg per lane A. marginale infected erythrocytes (lanes 1 to 4), whole A. marginale organisms (lanes 5 to 8), and purified A. marginale outer membranes (lanes 9 to 12) were separated using SDS-PAGE and transferred to a nitrocellulose membrane. MAb ANAF16 (A), reactive with MSP5, and MAb ANA8A (B), reactive with bovine erythrocyte membranes, were used to probe the samples for MSP5 enrichment and bovine erythrocyte contamination. Binding was detected using alkaline phosphatase-labeled anti-mouse antibody followed by chemiluminescence.