TABLE 2.
Comparative amino acid sequence analysis of newly identified A. marginale proteins and homologs in related organisms
| Protein annotation | BLAST identity and scorea | Organism identityb |
|---|---|---|
| Undefined product (AM197) | Predicted membrane protein (1e-24) | E. canis (YP_303304) |
| Undefined product (AM366) | No significant BLAST score | |
| Undefined product (AM854) | OMP and peptidoglycan associated protein (5e-30) | E. canis (YP_303196) |
| Undefined product (AM387) | No significant BLAST score | |
| Undefined product (AM127) | Hypothetical protein (1e-77) | E. chaffeensis (ZP_00544751) |
| Undefined product (AM529) | No significant BLAST score | |
| Undefined product (AM072) | No significant BLAST score | |
| OpAG2 (AM1142) | 2e-23 | E. chaffeensis (AAK28661) |
| OMP4 (AM1164) | 6e-32 | A. ovis (AAG37873) |
| OMP7 (AM1220) | No significant BLAST score | |
| OMP10 (AM1223) | No significant BLAST score | |
| OMP14 (AM075) | 8e-40 | A. phagocytophilum (AAO30097) |
| Conjugal transfer protein (AM097) | 2e-96 | E. canis (YP_303416) |
| VirB9 (AM1315) | 2e-80 | A. phagocytophilum (AAM00421) |
| VirB10 (AM1314) | 1e-118 | A. phagocytophilum (AAM00418) |
| Elongation factor Tu (AM254) | 0.0 | E. ruminantium (YP_180474) |
| OMA87 (AM1096) | 0.0 | E. chaffeensis (ZP_00544838) |
| PepA cytosol amino peptidase (AM956) | 2e-173 | E. ruminantium (CAI28112) |
| Anaplasma appendage-associated protein (AM878) | No significant BLAST score | |
| Anaplasma appendage-associated protein (AM879) | No significant BLAST score | |
| Anaplasma appendage-associated protein (AM880) | No significant BLAST score |
a
BLAST searches were performed on undefined and annotated proteins to determine the percent sequence identity with proteins in closely related organisms. BLAST E values of <1e-20 were considered to be significant and are indicated.
b
GenBank accession numbers of the proteins identified in related organisms are provided in parentheses.