TABLE 6.

SEC neutralization assay for the sera from conjugates

Serum no.a	Avg cpm ± SD	Neutralization (%)b	IgG (EU)
1	8,847 ± 1246	91.7	20.26
2	18,021 ± 1,831	83.2	21.92
3	10,068 ± 3,740	90.6	27.02
4	4,385 ± 98	95.9	15.16
5	52,684 ± 4,889	50.8	18.37
6	96,793 ± 82	9.6	5.71
7	75,315 ± 936	29.6	10.19
8	48,505 ± 2,358	54.7	13.45
9	82,623 ± 36	22.8	10.40
10	97,296 ± 4,173	9.1	7.39
11	64,437 ± 7,724	39.8	55.60
12	5,577 ± 2,778	94.8	100.96
13	71,770 ± 1,840	32.9	60.12
14	46,801 ± 4,074	56.3	113.57
Control (−)	107,023 ± 2,778	0.0	<0.01
Control (+)	2,448 ± 224	97.7	NDc

^aSera 1 to 5 were obtained from the third injection of W135P(S)-rSEC8 (rSEC was coupled with the sonicated W135P). Sera 6 to 11 were obtained after the second injection of the bivalent conjugate (GAMP-rSEC3 + W135P(H)-rSEC6). Sera 12 to 14 were from the third injection of the bivalent conjugate. Serum in the control (−) was from a normal mouse with no detectable antibodies specific for SEC. Control (+) was a hyperimmune serum from a rabbit immunized with SEC.

^bThe neutralization percent was calculated by comparing the average cpm's in sera of immunized mice with the cpm in control (−).

^cND, not determined.