FIG. 5.

Enhanced IFN-γ and TNF-α production in splenocytes from mice coinfected with M. tuberculosis and P. yoelii 17XL compared to that in splenocytes from mice infected with P. yoelii 17XL alone, as measured by ELISA. Five mice per group were sacrificed at each time point. IFN-γ secretion by unstimulated splenocytes (A) and splenocytes stimulated with PHA (B), old tuberculin (C), P. yoelii lysate (D), and TNF-α secretion by unstimulated (E) and PHA-stimulated (F) splenocytes was measured in the supernatants collected after 24 h of incubation. Mtb, mice infected with M. tuberculosis; Py17XL, mice infected with P. yoelii 17XL; Mtb & Py17XL, mice infected with M. tuberculosis and P. yoelii 17XL. PI, postinfection.