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Transcription of fnbA is enhanced in mutants lacking agr, ClpX, or ClpP. RNA was isolated from cells in the mid-exponential growth phase (OD600 = 0.8 ± 0.1). Lane 1, 8325-4 (wild-type); lane 2, 8325-4 ΔclpP; lane 3, DF2268 (ΔclpP Δagr); lane 4, DF2269 (ΔclpX Δagr); lane 5, PC1839 (sarA); lane 6, DF2271 (ΔclpP sarA); lane 7, DF2308 (Δagr sarA); lane 8, RN6911 (Δagr); lane 9, 8325-4 ΔclpX. wt, wild type.
