TABLE 1.
Median antibody titers after three immunizations of C57BL/6 mice with recombinant MSP2 and MSP119 proteins
| Immunizing antigen | Median midpoint titer (105) (95% CI)a
|
Mean IgG1/IgG2b ratiob | P (MSP2 vs MSP119) | ||||
|---|---|---|---|---|---|---|---|
| Total IgG | IgG1 | IgG2a | IgG2b | IgG3 | |||
| MSP119 | 8.34 (2.19) | 5.02 (1.97) | 0.08 (0.41) | 3.89 (0.52) | 0.05 (0.13) | 2.89 | |
| Serogroup A | |||||||
| Full length (MSP2A) | 9.60 (1.79) | 6.34 (1.66) | 0.83 (0.44) | 2.95 (0.37) | 0.43 (0.18) | 4.69 | 0.008 |
| Di-A | 17.8 (3.57) | 2.55 (1.43) | 0.18 (0.09) | 12.0 (3.09) | 0.11 (0.08) | 0.32 | 0.007 |
| Poly-A | 0.64 (0.30) | 0.19 (0.12) | 0.04 (0.02) | 0.47 (0.12) | 0.03 (0.02) | 0.51 | 0.011 |
| Serogroup B | |||||||
| Full length (MSP2B) | 19.3 (4.55) | 11.8 (2.58) | 0.78 (0.41) | 3.91 (1.38) | 0.51 (0.13) | 5.92 | 0.014 |
| Di-B | 3.84 (1.89) | 0.76 (0.47) | 0.04 (0.01) | 2.50 (1.25) | 0.03 (0.03) | 0.34 | 0.009 |
| Poly-B | 5.5 (0.63) | 1.11 (0.88) | 0.07 (0.05) | 4.37 (1.25) | 0.03 (0.01) | 0.44 | 0.008 |
| Con-C | 2.61 (0.28) | 0.18 (0.12) | 0.08 (0.06) | 2.22 (0.63) | 0.06 (0.03) | 0.18 | 0.006 |
| Con-N | 0.64 (0.11) | 0.45 (0.08) | 0.02 (0.01) | 0.42 (0.10) | 0.01 (0.01) | 1.46 | 0.064 |
a
There were six mice per group. 95% CI, 95% confidence interval.
b
Calculated as the mean for each mouse. Shown are the mean ratios of IgG1 to IgG2b (the IgG1/IgG2b index) and comparisons between the IgG1/IgG2b index for MSP2 proteins and the index for MSP119 (Wilcoxon rank sum tests).