TABLE 2.
Lymphoproliferative responsesa of splenocytes from immunized C57BL/6 mice to recombinant protein and synthetic peptide antigens
| Immunizing antigen | SI for indicated antigen for restimulation of spleen cellsb
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Poly-A | Con-C | MSP119 | C1 | C2 | C3 | C4 | C5 | C6 | C7 | C8 | L | GST | |
| MSP2A | 4.4 | 4.4 | 1.3 | 0.5 | 0.9 | 0.8 | 1.1 | 0.5 | 1.8 | 1.1 | 2.0 | 1.1 | 1.1 |
| 8.1 | 5.9 | 1.0 | 0.4 | 1.1 | 1.5 | 0.4 | 0.3 | 1.3 | 4.5 | 7.4 | 1.3 | 1.7 | |
| 5.1 | 4.0 | 1.2 | 0.7 | 1.0 | 1.3 | 1.1 | 0.8 | 1.4 | 1.4 | 3.0 | 1.4 | 1.2 | |
| 8.7 | 5.0 | 1.4 | 0.3 | 1.0 | 1.2 | 0.6 | 0.4 | 1.1 | 1.0 | 6.3 | 0.9 | 1.1 | |
| 6.5 | 3.1 | 1.2 | 1.6 | 5.3 | 6.4 | 1.6 | 1.5 | 2.3 | 9.0 | 14 | 3.0 | 3.2 | |
| Mean SI | 6.6 | 4.5 | 1.2 | 0.7 | 1.9 | 2.2 | 1.0 | 0.7 | 1.6 | 3.4 | 6.5 | 1.5 | 1.7 |
| SE | 1.9 | 1.1 | 0.2 | 0.5 | 1.9 | 2.3 | 0.5 | 0.5 | 0.5 | 3.5 | 4.7 | 0.8 | 0.9 |
| Con-C | 7.1 | 6.1 | 2.1 | 0.8 | 1.1 | 1.5 | 1.2 | 1.0 | 1.1 | 1.8 | 3.1 | 1.2 | 2.0 |
| 7.8 | 4.6 | 1.3 | 0.4 | 1.1 | 2.0 | 0.6 | 0.5 | 0.8 | 1.2 | 2.1 | 1.1 | 2.1 | |
| 2.0 | 0.9 | 0.6 | 0.6 | 0.8 | 0.3 | 0.8 | 0.2 | 0.1 | 1.4 | 0.8 | 0.5 | 0.8 | |
| 9.1 | 5.1 | 1.1 | 1.0 | 0.8 | 2.1 | 1.1 | 0.8 | 1.6 | 2.1 | 4.2 | 3.0 | 1.1 | |
| Mean SI | 6.5 | 4.2 | 1.3 | 0.7 | 1.0 | 1.5 | 0.9 | 0.6 | 0.9 | 1.6 | 2.6 | 1.5 | 1.5 |
| SE | 3.1 | 2.3 | 0.6 | 0.3 | 0.3 | 0.8 | 0.3 | 0.4 | 0.4 | 0.4 | 1.5 | 1.1 | 0.7 |
| MSP119 | 1.2 | 1.0 | 4.8 | 1.0 | 1.0 | 1.6 | 1.1 | 0.6 | 1.2 | 1.6 | 1.8 | 1.0 | 1.1 |
| 3.9 | 2.1 | 9.1 | 0.4 | 0.9 | 1.3 | 0.5 | 0.7 | 1.0 | 1.3 | 3.0 | 2.8 | 1.8 | |
| 1.8 | 1.8 | 1.8 | 1.1 | 1.1 | 0.6 | 1.1 | 0.9 | 0.9 | 1.0 | 1.0 | 1.6 | 0.6 | |
| 1.1 | 0.8 | 3.2 | 1.1 | 0.9 | 1.2 | 1.2 | 1.1 | 1.1 | 1.6 | 2.0 | 1.2 | 1.0 | |
| 0.3 | 0.7 | 0.6 | 0.3 | 1.1 | 0.5 | 0.7 | 0.4 | 0.5 | 0.9 | 0.4 | 0.4 | 0.8 | |
| Mean SI | 1.7 | 1.3 | 3.9 | 0.8 | 1.0 | 1.0 | 0.9 | 0.7 | 0.9 | 1.3 | 1.6 | 1.4 | 1.1 |
| SE | 1.4 | 0.6 | 3.3 | 0.4 | 0.1 | 0.5 | 0.3 | 0.3 | 0.3 | 0.3 | 1.0 | 0.9 | 0.5 |
Splenocytes from immunized C57BL/6 mice were cultured for 6 days with MSP2-A or MSP119 antigen (1 μg/ml) or peptide (10 μg/ml). Cultures were pulsed with [3H]thymidine on day 5 and harvested on day 6.
SI, stimulation indices. SI were determined as the geometric mean number of cpm of antigen-stimulated cells divided by the geometric mean number of cpm of medium control cells. Numbers of cpm for medium control cells ranged between 24 cpm and 274 cpm. Boldface values indicate SI values of ≥2. Peptide sequences were as follows: for C1, AENSAPTAEQTESPELQSAPC; for C2, TESPELQSAPENKGTGQHGHC; for C3, ENKGTGQHGHMHGSRNNHPQC; for C4, MHGSRNNHPQNTSDSQKECTC; for C5, NTSDSQKECTDGNKENCGAAC; for C6, DGNKENCGAATSLLNNSS; for C7, TSLLNNSSNIASINKFVVLIC; for C8, ASINKFVVLISATLVLSFAIC; and for L, FSPFLNFFMVKFSPFLNFFMVK.