FIG. 5.
(A) Kinetics of the humoral response elicited after immunization with Omp3148-74. Mice were immunized with Omp3148-74 or rOmp31 in incomplete Freund's adjuvant and bled retroorbitally on the indicated days. IgG-specific Abs against rOmp31 (dashed lines) or Omp3148-74 (solid lines) were evaluated by ELISA. Each symbol represents the mean ± SD of eight animals. Determination of IFN-γ (B) or IL-2 (C) production in cells from PBS- or Omp3148-74-immunized mice. Splenocytes were depleted of CD4+ T cells (-CD4+) or CD8+ T cells (-CD8+) using mouse CD4 (L3T4) Dynabeads or mouse CD8 (Lyt2) Dynabeads or were not depleted (T). Cells (4 × 106/ml) from mice were stimulated with complete medium (RPMI) or rOmp31 (1 μg/ml) for 48 h. IFN-γ or IL-2 in cell supernatants was quantified (pg/ml) by MAb capture ELISA. Each value represents the mean plus SD of the responses of spleen cells from five individual mice. The data are representative of two separate experiments. ★, significantly different from the same stimulus in PBS-immunized mice (P < 0.01).