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. 2002 Sep;40(9):3326–3333. doi: 10.1128/JCM.40.9.3326-3333.2002

FIG. 2.

FIG. 2.

Agarose gel electrophoresis of restriction endonuclease-digested plasmid DNAs and hybridization with plasmid pMG1. (A1) Agarose gel electrophoresis of EcoRI-digested plasmid DNAs isolated from gentamicin-resistant E. faecium BM4105SS transconjugants harboring the type A gentamicin resistance conjugative plasmid. (A2) The gel was Southern blotted and hybridized to pMG1. (B1) Agarose gel electrophoresis of EcoRI-digested plasmid DNA isolated from gentamicin-resistant E. faecium BM4105SS transconjugants harboring the type B gentamicin resistance conjugative plasmid. (B2) The gel was Southern blotted and hybridized to pMG1. (A) Lanes: 1, HindIII-digested lambda DNA; lanes 2 to 15, EcoRI-digested plasmid DNA isolated from transconjugants of strains 161, 200, 210, 253, 300, 306, 311, 317, 376, 469, 494, 537, 581, and 692, respectively; lane 16, EcoRI-digested pMG1. (B) Lanes: 1, HindIII-digested lambda DNA; lanes 2 to 14, EcoRI-digested plasmid DNA isolated from transconjugants of strains 70, 87, 133, 166, 205, 247, 282, 411, 445, 526, 587, 619, 666, and 725, respectively; lane 15, EcoRI-digested pMG1.