TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or phenotype | Description and/or reference(s) |
|---|---|---|
| Strains | ||
| E. faecium BM4147 (pIP816 Vanr) | van | 29 |
| E. faecalis FA2-2 | rif fus | Derivative of JH2; 10 |
| E. faecalis JH2SS | str spc | Derivative of JH2: 39 |
| E. faecium BM4105RF | rif fus | Derivative of plasmid-free E. faecium BM4105; 5 |
| E. faecium BM4105SS | str spc | Derivative of plasmid-free E. faecium BM4105; 5 |
| Plasmids | ||
| pMG1 | Gmr | 65.1-kb conjugative plasmid from E. faecium strain; 26 |
| pAD1 | hly/bac uvr | 59.6-kb pheromone-responsive conjugative plasmid from DS16; 39 |
| pPD1 | bac | 59-kb pheromone-responsive conjugative plasmid from E. faecalis 39-5; 19, 42 |
| pAM373 | tet | 36-kb pheromone-responsive conjugative plasmid; 9 |
| pAMβ1 | erm | 26.5-kb broad-host-range conjugative plasmid from DS5; 28 |
| pIP501 | erm cat | 39.2-kb broad-host-range conjugative plasmid; 17 |