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Immunoblot assays with purified lipooligosaccharide of M. catarrhalis strains (noted at the top). Immunoblots were probed with homologous preacquisition sputum supernatants (lanes a) and postclearance sputum supernatants (lanes b). Antibodies were detected with peroxidase-conjugated anti-human IgA. Molecular masses are noted in kilodaltons on the right. The arrow denotes the presence of IgA binding to lipooligosaccharide in the postclearance sputum sample.
