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. 2005 Dec;73(12):8161–8166. doi: 10.1128/IAI.73.12.8161-8166.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 4. — Immunoblot assays with purified IgA from postclearance sputum supernatant samples corresponding to strain 7P94B1 (top panel) and strain 44P23B1 (bottom panel). Lanes contain purified outer membrane preparations of the following strains: the homologous strain (lane a), O35E (lane b), the UspA1 mutant (lance c), the UspA2 mutant (lane d), the UspA1 UspA2 double mutant (lane e), the CopB mutant (lane f), the TbpB mutant (lane g), the Hag mutant (lane h), and the UspA1 UspA2 Hag triple mutant (lane i). Antibodies were detected with peroxidase-conjugated anti-human IgA. Molecular mass markers are noted on the left in kilodaltons. Arrows denote the locations of UspA2, Hag, UspA1, and UspA2 monomers.