TABLE 2.

Summary of outer membrane antigens to which sputum IgA is directed following M. catarrhalis carriage in adults with COPD

Patient visit IDb no.	Clinical episodec	Duration of carriage (mo)d	Presence of IgA band and relative densities of bands in preacquisition/postclearance sputum samples fora:
			UspA1	UspA2	Hag	TbpB	CopB	LOS
1PS45	Col	3	+, 46.8/53.2		+, 46.4/53.6
3PS68	Col	1			+, 37.6/62.4	+, 27.7/72.3
7PS95	Exac	1	+, 34.8/65.2	+, 33.5/66.5	+, 44.5/55.5			+
10PS92	Exac	1	+, 53.0/47.0		+, 52.9/47.1	+, 56.4/43.6
12PS75	Exac	2	+, 19.1/80.9	+, 13.0/87.0	+, 19.4/80.6		+, 42/58
19PS8	Col	1	+e	+e	+, 54.1/45.9
25PS26	Col	1	+, 14.8/85.2	+, 32.9/67.1	+, 37.5/62.5
29PS25	Col	1	+, 54.0/46.0	+, 54.0/46.0	+f
44PS24	Col	1	+, 30.1/69.9	+, 28.1/71.9	+, 31.6/68.4
66PS6	Exac	1	+, 30.9/69.1	+, 30.9/69.1	+, 33.1/66.9	+, 27.1/72.9
Total			9/10	7/10	10/10	3/10	1/10	1/10

^aA “+” indicates the presence of a band in the immunoblot assay with IgA purified from the postclearance sample. Numbers after the symbol indicate the relative densities of bands in the immunoblot assay based on the integrated density values of IgA purified from preacquisition/postclearance sputum samples at identical concentrations and assayed with the homologous infecting strain. Bands with relative band densities of ∼52/48 or greater are different in intensity by visual inspection. Values of ∼70/30 or greater are essentially the development of a new band in the postclearance sample compared to the bands in the preacquisition sample by visual inspection.

^bID, identification.

^cCol, colonization defined by sputum that is culture positive for a newly acquired isolate of M. catarrhalis with no change in symptoms; Exac, exacerbation defined by sputum that is culture positive for a newly acquired isolate of M. catarrhalis from a patient with clinical symptoms of an exacerbation.

^dDetermined by monthly sputum cultures and molecular typing of isolates of M. catarrhalis.

^eAntibody to the antigen producing the band is present in postclearance sputum IgA, but there was no increase from the level in preacquisition sputum IgA.

^fRecognizes the band in strain O35E but not in the homologous isolate.